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Principal Investigators
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Prof. Dr.rer.nat. Barbara Walzog
Human neutrophils are well known to play an important role in host defense and inflammation. Adhesion molecules of the ß2 integrin (CD11/CD18) family are critically involved in the recruitment and activation of neutrophils during inflammation by mediating e.g. firm adhesion, intraluminal crawling and phagocytosis of opsonized particles. Ligand binding of the ß2 integrins activates the non-receptor tyrosine kinase Syk which plays an important role for neutrophil activation in inflammatory settings. Our group focusses on Syk-mediated signalling events required for leukocyte activation at the molecular level using different techniques including real-time analysis of live cells. By integrating studies on the molecular and cellular level as well as animal studies, this project is intended to improve the understanding of the spatial and temporal dynamics of neutrophil activation in a (patho-) physiological context which may lead to the identification of new molecular targets for therapeutic intervention.

Members of the Walzog Team: 

Begandt Daniela, Dr.
Brechtefeld Doris, Dipl. Biol.
Geß Sebastian, FöFoLe-Student
Hepper Ingrid, Dipl. Biol.
Jakob Sascha
Kemme Tobias, Dr.
Lamadé Eva
Pick Robert, Dr.
Salvermoser Melanie, MSc
Stocker Thomas
Vlaovic Tanja
Weckbach Ludwig
Winkelmann Michael
Zehrer Annette, MSc

Lab Technician:
Gylstorff Severin
Truong Jennifer

Recent publications:

Lorenz V, Stegner D, Stritt S, Vögtle T, Kiefer F, Witke W, Schymeinsky J, Watson SP, Walzog B, Nieswandt B. Targeted downregulation of platelet CLEC-2 occurs through Syk-independent internalization. Blood. 2015 Mar 20. pii: blood-2014-11-611905. [Epub ahead of print]

Weckbach LT, Gola A, Winkelmann M, Jakob SM, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Müller-Höcker J, Deindl E, Sperandio M, Walzog B. (2014) The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via ß2 integrins (CD11/CD18). Blood 2014 Mar 20; 123(12):1887-96

Pick R, Brechtefeld D, Walzog B. Intraluminal crawling versus interstitial neutrophil migration during inflammation. Mol Immunol. 2013 Aug; 55(1) :70-5;

Jakob SM, Pick R, Brechtefeld D, Nussbaum C, Kiefer F, Sperandio M, Walzog B. Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1-mediated neutrophil recruitment during the acute inflammatory response. Blood. 2013 May 16; 121(20):4184-94

Stark K, Eckart A, Haidari S, Tirniceriu A, Lorenz M, von Brühl ML, Gärtner F, Khandoga AG, Legate KR, Pless R, Hepper I, Lauber K, Walzog B, Massberg S. Capillary and arteriolar pericytes attract innate leukocytes exiting through venules and 'instruct' them with pattern-recognition and motility programs. Nat Immunol. 2013 Jan; 14(1):41-51

Weckbach LT, Groesser L, Borgolte J, Pagel JI, Pogoda F, Schymeinsky J, Müller-Höcker J, Shakibaei M, Muramatsu T, Deindl E, Walzog B. Midkine acts as proangiogenic cytokine in hypoxia-induced angiogenesis. Am J Physiol Heart Circ Physiol. 2012 Aug 15; 303(4):H429-38

Futosi K, Németh T, Pick R, Vántus T, Walzog B, Mócsai A. Dasatinib inhibits proinflammatory functions of mature human neutrophils. Blood. 2012 May 24; 119(21):4981-91

Hepper I, Schymeinsky J, Weckbach LT, Jakob SM, Frommhold D, Sixt M, Laschinger M, Sperandio M, Walzog B. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol. 2012 May 1; 188(9):4590-601

Weckbach LT, Muramatsu T, Walzog B. Midkine in inflammation. ScientificWorldJournal. 2011 Dec 27; 11:2491-505

Schymeinsky J, Sperandio M, Walzog B. The mammalian actin-binding protein 1 (mAbp1): a novel molecular player in leukocyte biology. Trends Cell Biol. 2011 Apr; 21(4):247-55

Németh T, Futosi K, Hably C, Brouns MR, Jakob SM, Kovács M, Kertész Z, Walzog B, Settleman J, Mócsai A. Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice. J Immunol. 2010 Sep 1; 185(5):3064-75

Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood. 2010 Aug 5; 116(5):841-9

Schymeinsky J, Gerstl R, Mannigel I, Niedung K, Frommhold D, Panthel K, Heesemann J, Sixt M, Quast T, Kolanus W, Mocsai A, Wienands J, Sperandio M, Walzog B. A fundamental role of mAbp1 in neutrophils: impact on beta(2) integrin-mediated phagocytosis and adhesion in vivo. Blood. 2009 Nov 5; 114(19):4209-20

Sindrilaru A, Peters T, Schymeinsky J, Oreshkova T, Wang H, Gompf A, Mannella F, Wlaschek M, Sunderkötter C, Rudolph KL, Walzog B, Bustelo XR, Fischer KD, Scharffetter-Kochanek K. Wound healing defect of Vav3-/- mice due to impaired {beta}2-integrin-dependent macrophage phagocytosis of apoptotic neutrophils. Blood. 2009 May 21; 113(21):5266-76

Mannell H, Hellwig N, Gloe T, Plank C, Sohn HY, Groesser L, Walzog B, Pohl U, Krotz F. Inhibition of the tyrosine phosphatase SHP-2 suppresses angiogenesis in vitro and in vivo. J Vasc Res. 2008; 45(2):153-63

Frommhold D, Mannigel I, Schymeinsky J, Mocsai A, Poeschl J, Walzog B, Sperandio M. Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo. BMC Immunol. 2007 Nov 28; 8:31

Schymeinsky J, Then C, Sindrilaru A, Gerstl R, Jakus Z, Tybulewicz VL, Scharffetter-Kochanek K, Walzog B. Syk-mediated translocation of PI3Kdelta to the leading edge controls lamellipodium formation and migration of leukocytes. PLoS One. 2007 Nov 7; 2(11):e1132

Schymeinsky J, Mócsai A, Walzog B. Neutrophil activation via beta2 integrins (CD11/CD18): molecular mechanisms and clinical implications. Thromb Haemost. 2007 Aug; 98(2):262-73