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Principal Investigators
Career Opportunities
Prof. Fritz Krombach

Using advanced imaging techniques, research in the Krombach lab is focussed on (i) the mechanisms regulating leukocyte trafficking in inflammatory disease (DFG SFB 914) and (ii) the distribution/fate and biological effects of nanomaterials at the microscopic level under physiologic and pathophysiologic (thrombosis, inflammation, ischemia-reperfusion) conditions (DFG SFB 1032, DFG SPP 1313, EU FP7 MARINA and EU FP7 NANOSOLUTIONS).


Members of the Krombach Team:

Reichel Christoph, PD (Gastarzt Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde)
Uhl Bernd, Dr. med. (Gastarzt Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde)

Lab Technicians:
Adams Gerhard
Fahney Claudia

Recent publications:

Uhl B, Hirn S, Immler R, Mildner K, Möckl L, Sperandio M, Bräuchle C, Reichel CR, Zeuschner D, Krombach F. The endothelial glycocalyx controls interactions of nanoparticles with the endothelium and their translocation across the blood-tissue border. ACS Nano 2017; 11:1498-1508.

Möckl L, Hirn S, Torrano AA, Uhl B, Bräuchle C, Krombach F. The glycocalyx regulates the uptake of nanoparticles by human endothelial cells in vitro. Nanomedicine (Lond) 2017; 12:207-217.

Sellner S, Kocabey S, Zhang T, Nekolla K, Hutten S, Krombach F, Liedl T, Rehberg M. Dexamethasone-conjugated DNA nanotubes as anti-inflammatory agents in vivo. Biomaterials 2017; 134:78–90.

Ganguly K, Ettehadieh D, Upadhyay S, Takenaka S, Adler T, Karg E, Krombach F, Kreyling WG, Schulz H, Schmid O, Stoeger T. Early pulmonary response is critical for extra-pulmonary carbon nanoparticle mediated effects: Comparison of inhalation versus intra-arterial infusion exposures in mice. Part Fibre Toxicol 2017;14:19.

Uhl B, Vadlau Y, Zuchtriegel G, Nekolla K, Sharaf K, Gaertner F, Massberg S, Krombach F, Reichel CA. Aged neutrophils contribute to the first line of defense in the acute inflammatory response. Blood 2016; 128:2327-2337.

Daniel JM, Reichel CA, Schmidt-Woell T, Dutzmann J, Zuchtriegel G, Krombach F, Herold J, Bauersachs J, Sedding DG, Kanse SM. Factor VII activating protease deficiency promotes neointima formation by enhancing leukocyte accumulation. J Thromb Haemost. 2016 Jul 19. [Epub ahead of print]

Nekolla K, Kick K, Sellner S, Mildner K, Zahler S, Zeuschner D, Krombach F, Rehberg M. Influence of surface modifications on the spatio-temporal microdistribution of quantum dots in vivo. Small 2016;12: 2641-2651.

Zuchtriegel G, Uhl B, Puhr-Westerheide D, Pörnbacher M, Lauber K, Krombach F, Reichel CA. Platelets guide leukocytes to their sites of extravasation. PLoS Biol 2016 May 6;14(5):e1002459.

Dietzel S, Hermann S, Kugel Y, Sellner S, Uhl B, Hirn S, Krombach F, Rehberg M. Multi-photon microscopy of non-fluorescent nanoparticles in vitro and in vivo. Small 2016;12: 3245-3257.

Rehberg M, Nekolla K, Sellner S, Praetner M, Mildner K, Zeuschner D, Krombach F. Intercellular transport of nanomaterials is mediated by membrane nanotubes in vivo. Small 2016;12: 1882-1890.

Sellner S, Kocabey S, Nekolla AK, Krombach F, Liedl T, Rehberg M. DNA Nanotubes as Intracellular Delivery Vehicles in vivo. Biomaterials 2015;53: 453-463.

Zuchtriegel G, Uhl B, Hessenauer ME, Kurz AR, Rehberg M, Lauber K, Krombach F, Reichel CA. Spatiotemporal Expression Dynamics of Selectins Govern the Sequential Extravasation of Neutrophils and Monocytes in the Acute Inflammatory Response. Arterioscler Thromb Vasc Biol. 2015 Feb 26. [Epub ahead of print]

Haberl N, Hirn S, Holzer M, Zuchtriegel G, Rehberg M, Krombach F. Effects of acute systemic administration of TiO2, SiO2, ZnO, and Ag nanoparticles on hemodynamics, hemostasis, and leukocyte recruitment. Nanotoxicology 2015 Feb 11:1-9.

Reichel CA, Hessenauer ME, Pflieger K, Rehberg M, Kanse SM, Zahler S, Krombach F, Berghaus A, Strieth S. Components of the Plasminogen Activation System Promote Engraftment of Porous Polyethylene Biomaterial via Common and Distinct Effects. PLoS One 2015;10:e0116883.

Hirn S, Haberl S, Loza K, Epple M, Kreyling WG, Rothen-Rutishauser B, Rehberg M, Krombach F. Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices. Beilstein J Nanotechnol 2014; 5:2440–2449.

Linkermann A, Skouta R, Himmerkus N, Mulay SR, Dewitz C, De Zen F, Prokai A, Zuchtriegel G, Krombach F, Welz PS, Weinlich R, Vanden Berghe T, Vandenabeele P, Pasparakis M, Bleich M, Weinberg JM, Reichel CA, Bräsen JH, Kunzendorf U, Anders HJ, Stockwell BR, Green DR, Krautwald S. Synchronized renal tubular cell death involves ferroptosis. Proc Natl Acad Sci U S A 2014; 111:16836-41.

Ahlberg S, Antonopulos A, Diendorf J, Dringen R, Epple M, Flöck R, Goedecke W, Graf C, Haberl N, Helmlinger J, Herzog F, Heuer F, Hirn S, Johannes C, Kittler S, Köller M, Korn K, Kreyling WG, Krombach F, Lademann J, Loza K, Luther EM, Malissek M, Meinke MC, Nordmeyer D, Pailliart A, Raabe J, Rancan F, Rothen-Rutishauser B, Rühl E, Schleh C, Seibel A, Sengstock C, Treuel L, Vogt A, Weber K, Zellner R. PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments. Beilstein J Nanotechnol 2014; 5:1944-1965.

Uhl B, Zuchtriegel G, Puhr-Westerheide D, Praetner M, Rehberg M, Fabritius M, Hessenauer M, Holzer M, Khandoga A, Fürst R, Zahler S, Krombach F, Reichel CA. Tissue plasminogen activator promotes postischemic neutrophil recruitment via its proteolytic and non-proteolytic properties. Arterioscler Thromb Vasc Biol 2014 Jul;34(7):1495-504.

Holzer M, Bihari P, Praetner M, Uhl B, Reichel C, Fent J, Vippola M, Lakatos S, Krombach F. Carbon-based nanomaterials accelerate arteriolar thrombus formation in the murine microcirculation independently of their shape. J Appl Toxicol 2014 Feb 14.

Mende K, Reifart J, Rosentreter D, Manukyan D, Mayr D, Krombach F, Rentsch M, Khandoga A. Targeting platelet migration in the postischemic liver by blocking protease-activated receptor 4. Transplantation 2014 Jan 27;97(2):154-60.

Haberl N, Hirn S, Wenk A, Diendorf J, Epple M, Johnston BD, Krombach F, Kreyling WG, Schleh C. Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats. Beilstein J. Nanotechnol. 2013;4: 933–940.

Lerchenberger M, Uhl B, Stark K, Zuchtriegel G, Eckart A, Miller M, Puhr-Westerheide D, Praetner M, Rehberg M, Khandoga AG, Lauber K, Massberg S, Krombach F, Reichel CA. Matrix metalloproteinases modulate ameboid-like migration of neutrophils through inflamed interstitial tissue. Blood 2013;122:770-80.

Collmann E, Bohnacker T, Marone R, Dawson J, Rehberg M, Stringer R, Krombach F, Burkhart C, Hirsch E, Hollingworth GJ, Thomas M, Wymann MP. Transient targeting of PI3K acts as roadblock in mast cells’ route to allergy. J Allergy Clin Immunol 2013; 2013 May 14 [Epub ahead of print]

Lech M, Römmele C, Gröbmayr R, Eka Susanti H, Kulkarni OP, Wang S, Gröne HJ, Uhl B, Reichel C, Krombach F, Garlanda C, Mantovani A, Anders HJ. Endogenous and  exogenous pentraxin-3 limits postischemic acute and chronic kidney injury. Kidney Int 2013;83:647-61.

Ittner A, Block H, Reichel CA, Varjosalo M, Gehart H, Sumara G, Gstaiger M, Krombach F, Zarbock A, Ricci R. Regulation of PTEN activity by p38δ-PKD1 signaling in neutrophils confers inflammatory responses in the lung. J Exp Med 2012;209:2229-46.

Reichel CA, Puhr-Westerheide D, Uhl B, Berberich N, Zahler S, Wymann MP, Luckow B, Krombach F. C-C motif chemokine CCL3 and canonical neutrophil attractants promote neutrophil extravasation through common and distinct mechanisms. Blood 2012;120:880-890.

Allam R, Scherbaum CR, Darisipudi MN, Mulay SR,  Hägele H, Lichtnekert J, Hagemann JH, Rupanagudi KV, Ryu M, Schwarzenberger C, Hohenstein B, Hugo C, Uhl B, Reichel CA, Krombach F, Monestier M, Moreth K, Schaefer L, Anders HJ. Histones from dying renal cells aggravate acute kidney injury via TLR-2 and -4. J Am Soc Nephrol 2012;23:1375-88.

Rehberg M, Leite CF, Mildner K, Horstkotte J, Zeuschner D, Krombach F. The surface chemistry of quantum dots determines their behavior in postischemic tissue. ACS Nano 2012;6:1370-1379.

Rehberg M, Krombach F, Pohl U, Dietzel S. Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. PLoS One 6(11): e28237.

Bubik MF, Willer EA, Bihari P, Jürgenliemk G, Ammer H, Krombach F, Zahler S, Vollmar AM, Fürst R. A novel approach to prevent endothelial hyperpermeability: The Crataegus extract WS® 1442 targets the cAMP/Rap1 pathway. J Mol Cell Cardiol 2011; 52:196-205.

Reichel CA, Lerchenberger M, Uhl B, Khandoga A, Rehberg M, Schmalix W, Deindl E, Lorenzl S, Kanse S, Krombach F. Urokinase-type plasminogen activator promotes paracellular transmigration of neutrophils via Mac-1, but independently of uPAR. Circulation 2011;124:1848-1859.

Mayer BA, Rehberg M, Erhardt A, Wolf A, Reichel CA, Kracht M, Krombach F, Tiegs G, Zahler S, Vollmar AM, Fürst R. Inhibitor of apoptosis proteins (IAPs) as novel targets in inflammatory processes. Arterioscler Thromb Vasc Biol 2011;31:2240-2250.

Berberich N, Uhl B, Joore J, Schmerwitz UK, Mayer BA, Reichel CA, Krombach F, Zahler S, Vollmar AM, Furst R. Roscovitine blocks leukocyte extravasation by inhibition of cyclin-dependent kinases 5 and 9. Br J Pharmacol 2011 July;163(5):1086-98.

Schmerwitz UK, Sass G, Khandoga AG, Joore J, Mayer BA, Berberich N, Totzke F, Krombach F, Tiegs G, Zahler S, Vollmar AM, Furst R. Flavopiridol protects against inflammation by attenuating leukocyte-endothelial interaction via inhibition of cyclin-dependent kinase 9. Arterioscler Thromb Vasc Biol 2011 February;31(2):280-8.

Reichel CA, Lerchenberger M, Uhl B, Rehberg M, Berberich N, Zahler S, Wymann MP, Krombach F. Plasmin inhibitors prevent leukocyte accumulation and remodeling events in the postischemic microvasculature. PLoS One 2011;6(2):e17229.

Rehberg M, Praetner M, Leite CF, Reichel CA, Bihari P, Mildner K, Duhr S, Zeuschner D, Krombach F. Quantum dots modulate leukocyte adhesion and transmigration depending on their surface modification. Nano Lett 2010 September 8;10(9):3656-64.

Praetner M, Rehberg M, Bihari P, Lerchenberger M, Uhl B, Holzer M, Eichhorn ME, Furst R, Perisic T, Reichel CA, Welsch U, Krombach F. The contribution of the capillary endothelium to blood clearance and tissue deposition of anionic quantum dots in vivo. Biomaterials 2010 September;31(26):6692-700.

Bixel MG, Li H, Petri B, Khandoga AG, Khandoga A, Zarbock A, Wolburg-Buchholz K, Wolburg H, Sorokin L, Zeuschner D, Maerz S, Butz S, Krombach F, Vestweber D. CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis. Blood 2010 August 19;116(7):1172-84.

Petri B, Broermann A, Li H, Khandoga AG, Zarbock A, Krombach F, Goerge T, Schneider SW, Jones C, Nieswandt B, Wild MK, Vestweber D. Von Willebrand factor promotes leukocyte extravasation. Blood 2010 August 17.

Khandoga A, Stoeger T, Khandoga AG, Bihari P, Karg E, Ettehadieh D, Lakatos S, Fent J, Schulz H, Krombach F. Platelet adhesion and fibrinogen deposition in murine microvessels upon inhalation of nanosized carbon particles. J Thromb Haemost 2010 July;8(7):1632-40.

Bihari P, Holzer M, Praetner M, Fent J, Lerchenberger M, Reichel CA, Rehberg M, Lakatos S, Krombach F. Single-walled carbon nanotubes activate platelets and accelerate thrombus formation in the microcirculation. Toxicology 2010 March 10;269(2-3):148-54.

Rehberg M, Krombach F, Pohl U, Dietzel S. Signal improvement in multiphoton microscopy by reflection with simple mirrors near the sample. J Biomed Opt 2010 March;15(2):026017.

Hagele H, Allam R, Pawar RD, Reichel CA, Krombach F, Anders HJ. Double-stranded DNA activates glomerular endothelial cells and enhances albumin permeability via a toll-like receptor-independent cytosolic DNA recognition pathway. Am J Pathol 2009 November;175(5):1896-904.

Lech M, vila-Ferrufino A, Allam R, Segerer S, Khandoga A, Krombach F, Garlanda C, Mantovani A, Anders HJ. Resident dendritic cells prevent postischemic acute renal failure by help of single Ig IL-1 receptor-related protein. J Immunol 2009; September 15;183(6):4109-18.

Reichel CA, Rehberg M, Lerchenberger M, Berberich N, Bihari P, Khandoga AG, Zahler S, Krombach F. Ccl2 and Ccl3 Mediate Neutrophil Recruitment via Induction of Protein Synthesis and Generation of Lipid Mediators. Arterioscler Thromb Vasc Biol 2009 July 16.

Khandoga AG, Khandoga A, Anders HJ, Krombach F. Postischemic vascular permeability requires both TLR-2 and TLR-4, but only TLR-2 mediates the transendothelial migration of leukocytes. Shock 2009 June;31(6):592-8.

Molteni R, Crespo CL, Feigelson S, Moser C, Fabbri M, Grabovsky V, Krombach F, Laudanna C, Alon R, Pardi R. {beta}-arrestin 2 is required for the induction and strengthening of integrin-mediated leukocyte adhesion during CXCR2-driven extravasation. Blood 2009; 114: 1073-82.

Khandoga A, Huettinger S, Khandoga AG, Li H, Butz S, Jauch KW, Vestweber D, Krombach F. Leukocyte transmigration in inflamed liver: A role for endothelial cell-selective adhesion molecule. J Hepatol 2009 April;50(4):755-65.

Cera MR, Fabbri M, Molendini C, Corada M, Orsenigo F, Rehberg M, Reichel CA, Krombach F, Pardi R, Dejana E. JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling. J Cell Sci 2009 January 15;122(Pt 2):268-77.

Khandoga AG, Khandoga A, Reichel CA, Bihari P, Rehberg M, Krombach F. In vivo imaging and quantitative analysis of leukocyte directional migration and polarization in inflamed tissue. PLoS One 2009;4(3):e4693.

Hanschen M, Zahler S, Krombach F, Khandoga A. Reciprocal activation between CD4+ T cells and Kupffer cells during hepatic ischemia-reperfusion. Transplantation 2008 September 15;86(5):710-8.

Furst R, Bubik MF, Bihari P, Mayer BA, Khandoga AG, Hoffmann F, Rehberg M, Krombach F, Zahler S, Vollmar AM. Atrial natriuretic peptide protects against histamine-induced endothelial barrier dysfunction in vivo. Mol Pharmacol 2008 July;74(1):1-8.

Annecke T, Kubitz JC, Langer K, Hilberath JM, Kahr S, Krombach F, Bittmann I, Rehm M, Kemming GI, Conzen PF. Lung injury following thoracic aortic occlusion: comparison of sevoflurane and propofol anaesthesia. Acta Anaesthesiol Scand 2008 May 12.

Reichel CA, Rehberg M, Bihari P, Moser CM, Linder S, Khandoga A, Krombach F. Gelatinases mediate neutrophil recruitment in vivo: evidence for stimulus specificity and a critical role in collagen IV remodeling. J Leukoc Biol 2008 April;83(4):864-74.

Bihari P, Vippola M, Schultes S, Praetner M, Khandoga AG, Reichel CA, Coester C, Tuomi T, Rehberg M, Krombach F. Optimized dispersion of nanoparticles for biological in vitro and in vivo studies. Part Fibre Toxicol 2008;5:14.

Woodfin A, Reichel CA, Khandoga A, Corada M, Voisin MB, Scheiermann C, Haskard DO, Dejana E, Krombach F, Nourshargh S. JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration. Blood 2007 September 15;110(6):1848-56.

Bixel MG, Petri B, Khandoga AG, Khandoga A, Wolburg-Buchholz K, Wolburg H, Marz S, Krombach F, Vestweber D. A CD99-related antigen on endothelial cells mediates neutrophil but not lymphocyte extravasation in vivo. Blood 2007 June 15;109(12):5327-36.

Ninichuk V, Khandoga AG, Segerer S, Loetscher P, Schlapbach A, Revesz L, Feifel R, Khandoga A, Krombach F, Nelson PJ, Schlondorff D, Anders HJ. The role of interstitial macrophages in nephropathy of type 2 diabetic db/db mice. Am J Pathol 2007 April;170(4):1267-76.