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Principal Investigators
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Dr. Eloi Montañez

Angiogenesis, the formation of new blood vessels from pre-existing ones, is required for organogenesis, homeostasis and immune response, and it contributes to the progression of many diseases including cancer. Angiogenesis involves several morphogenetic steps, during which endothelial cells (ECs) polarize, migrate, establish cell-cell contacts and form vessel lumes. These processes all critically dependent on the organization and dynamic rearrangement of the endothelial actin cytoskeleton. We aim to understand, at the molecular level but in context of living organism, the mechanisms that regulate rearrangement of endothelial actin cytoskeleton during angiogenesis. Our current work focuses on signaling processes regulated by interaction between ECs and extracellular matrix.


Members of the Montanez Team: 
Fraccaroli Alessia

Lab Technicians:
Bergner Brigitte

Recent publications:

Spuul P, Daubon T, Pitter B, Alonso F, Fremaux I, Kramer I, Montanez E*, Génot E* VEGF-A/Notch-Induced Podosomes Proteolyse Basement Membrane Collagen-IV during Retinal Sprouting Angiogenesis. Cell Rep. 2016 17:484-500.
(*) corresponding author

Niels Heemskerk, Lilian Schimmel, Chantal Oort, Taofei Yin, Bin Ma, Kobus van Unen, Bettina Pitter, Stephan Huveneers, Joachim Goedhart, Yi Wu, Eloi Montanez, Abigail Woodfin, Jaap D. van Buul. F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signaling in vivo. Nat. Commun. 2015; in press

Alessia Fraccaroli, Bettina Pitter, Abdallah Abu Taha, Jochen Seebach, Stephan Huveneers, Julian Kirsch, Ricardo P. Casaroli-Marano, Stefan Zahler, Ulrich Pohl, Holger Gerhardt, Hans-J Schnittler and Eloi Montanez. Endothelial alpha-parvin controls integrity of developing vasculature and is required for maintenance of cell-cell junctions. Circ. Res. 2015; 117: 29-40.

Zhang L, Orban M, Lorenz M, Barocke V, Braun D, Urtz N, Christian Schulz, von Brühl ML, Tirniceriu A, Gaertner F, Proia RL, Graf T, Bolz SS, Montanez E, Prinz M, Müller A, von Baumgarten L, Billich A, Sixt M, Fässler R, von Andrian UH, Junt T and Massberg S. (2012) A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis. J Exp Med. doi: 10.1084/jem.20121090

Fraccaroli A, Franco CA, Rognoni E, Neto F, Rehberg M, Aszodi A, Wedlich-Söldner R, Pohl U, Gerhardt H and Montanez E. Visualization of endothelial actin cytoskeleton in the mouse retina. PLoS One 2012; 7(10): e47488. doi:10.1371/journal.pone.0047488

Montanez E*, Karaköse E, Tischner D, Villunger A and Fässler R*. PINCH-1 promotes Bcl-2-dependent survival signalling and inhibits JNK-mediated apoptosis in the primitive endoderm. J. Cell Sci. 2012; In press. (*) corresponding author

Rohwedder I*, Montanez E*, Beckmann K, Bengtsson E, Dunér P, Nilsson J, Soehnlein O and Fässler R. Plasma fibronectin deficiency impedes atherosclerosis progression and fibrous cap formation. EMBO Mol. Med. 2012; 4:564-76 (*) equal contribution

Wickström SA, Lange A, Montanez E, Fässler R. The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase! EMBO J. 2010; 29:281-91.

Montanez E, Wickström SA, Altstätter J, Chu H and Fässler R. Alpha-parvin controls vascular mural cell recruitment to vessel wall by regulating RhoA/ROCK signalling. EMBO J. 2009; 28: 3132-44.

Egea J, Erlacher C, Montanez E, Burtscher I, Yamagishi S, Hess M, Hampel F, Sanchez R, Rodriguez-Manzaneque MT, Bösl MR, Fässler R, Lickert H, and Klein R. Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesodermal differentiation. Genes & Dev. 2008; 22: 3349-3362.

Montanez E, Ussar S, Schifferer M, Bösl M, Zent R, Moser M, Fässler R. Kindlin-2 controls bidirectional signaling of integrins. Genes & Dev. 2008; 22: 1325-1330.

Wang HV, Chang LW, Brixius K, Wickström SA, Montanez E, Thievessen I, Schwander M, Müller U, Bloch W, Mayer U, Fässler R. Integrin-linked kinase stabilizes myotendinous junctions and protects muscle from stress-induced damage. J Cell Biol. 2008; 180:1037-49.

Montanez E, Piwko-Czuchra A, Bauer M, Li S, Yurchenco P, Fässler R. Analysis of integrin functions in peri-implantation embryos, hematopoietic system, and skin. Methods Enzymol. 2007; 426:239-89.

Legate KR, Montanez E, Kudlacek O and Fässler R. ILK, PINCH and parvin: the tIPP of integrin signalling. Nat. Rev. Mol. Cell Biol. 2006; 7: 20-31.

Berndt C, Montanez E, Villena J, Fabre M, Vilaro S, Reina M. Influence of cytoplasmic deletions on the filopodia-inducing effect of syndecan-3. Cell Biol Int. 2004; 11:829-33.

Montanez E, Casaroli-Marano RP, Vilaro S, Pagan R. Comparative study of tube assembly in three-dimensional collagen matrix and on Matrigel coats. Angiogenesis 2002; 5:167-172.