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Principal Investigators
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PD Dr.rer.nat. Steffen Dietzel

Light microscopy is an important technique for many life science research areas, including the biology of blood vessels and inflammation. We keep the central microscopes of the institute running and develop new applications in particular in multi-photon microscopy. Among other approaches, we use Third Harmonic Generation microscopy for the label-free intravital visualization of blood vessels and blood cells.

Members of the Dietzel Team:

Judenmann Dagmar
Kugel Yan
Wachal Katarina

Recent publications:

Dietzel S, Pircher J, Nekolla AK, Gull M, Brandli AW, Pohl U, Rehberg M. Label-free determination of hemodynamic parameters in the microcirculaton with third harmonic generation microscopy. PLoS One 2014;9:e99615.

Ziegler T, Horstkotte J, Schwab C, Pfetsch V, Weinmann K, Dietzel S, Rohwedder I, Hinkel R, Gross L, Lee S, Hu J, Soehnlein O, Franz WM, Sperandio M, Pohl U, Thomas M, Weber C, Augustin HG, Fassler R, Deutsch U, Kupatt C. Angiopoietin 2 mediates microvascular and hemodynamic alterations in sepsis. J Clin Invest 2013;123:3436–3445.

Direnberger S, Mues M, Micale V, Wotjak CT, Dietzel S, Schubert M, Scharr A, Hassan S, Wahl-Schott C, Biel M, Krishnamoorthy G, Griesbeck O. Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. Nat Commun. 2012; 28(3)1031. doi: 10.1038/ncomms2035.

Nagel J, Gross B, Meggendorfer M, Preiss C, Grez M, Brack-Werner R, Dietzel S. Stably integrated and expressed retroviral sequences can influence nuclear location and chromatin condensation of the integration locus. Chromosoma. 2012; 121(4):353-67.

Rehberg M, Krombach F, Pohl U, Dietzel S. Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. PLoS One. 2011;6(11):e28237.

Rehberg M, Krombach F, Pohl U, Dietzel S. Signal improvement in multiphoton microscopy by reflection with simple mirrors near the sample. J Biomed Opt 2010 March;15(2):026017.

Rienzo M, Nagel J, Casamassimi A, Giovane A, Dietzel S, Napoli C. Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells. Biochim Biophys Acta 2010 July;1799(7):487-95.

Hepperger C, Mayer A, Merz J, Vanderwall DK, Dietzel S. Parental genomes mix in mule and human cell nuclei. Chromosoma 2009 June;118(3):335-47.

Hable V, Greubel C, Bergmaier A, Reichart P, Hauptner A, Krücken R, Strickfaden H, Dietzel S, Cremer T, Drexler GA, Friedl AA, Dollinger G. The live cell irradiation- and observation setup at SNAKE. Nucl Instr and Meth in Phys Res B 2009 March 13;267(12-13):2090-7.

Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krucken R, Cremer T, Friedl AA, Dollinger G. Quantitative analysis of DNA-damage response factors after sequential ion microirradiation. Radiat Environ Biophys 2008 November;47(4):415-22.

Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krucken R, Cremer T, Dollinger G, Friedl AA. Competition effect in DNA damage response. Radiat Environ Biophys 2008 November;47(4):423-9.

Hepperger C, Mannes A, Merz J, Peters J, Dietzel S. Three-dimensional positioning of genes in mouse cell nuclei. Chromosoma 2008 December; 117(6):535-51, Epub 2008 July 3.

Poser I, Sarov M, Hutchins JR, Heriche JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA. BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods 2008 May;5(5):409-15.

Kim IH, Nagel J, Otten S, Knerr B, Eils R, Rohr K, Dietzel S. Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining. BMC Biotechnol 2007;7:92.

Hepperger C, Otten S, von HJ, Dietzel S. Preservation of large-scale chromatin structure in FISH experiments. Chromosoma 2007 April;116(2):117-33.